《植物生理学报》 2018, 54(5): 819-826

BHT对苹果果实轮纹病的防效及防御酶活性的影响

于春蕾, 孟璐璐, 练森, 李保华, 梁文星, 王彩霞^*

青岛农业大学植物医学学院, 山东省植物病虫害综合防控重点实验室, 山东省应用真菌重点实验室, 山东青岛266109

通信作者：王彩霞；E-mail: cxwang@qau.edu.cn

摘　要：

以‘富士’苹果果实为材料, 采用刺伤接种法, 测定了2,6-二叔丁基-4-甲基苯酚(BHT)对苹果果实轮纹病的防效及其防病机制。结果表明: 0.1 mmol·L^-1的BHT处理后间隔48~72 h接种轮纹病菌, 其防效最高达74%以上, 而该浓度BHT对轮纹病菌菌丝生长和分生孢子萌发均无明显的抑制作用。BHT处理后接种或不接种轮纹病菌, 果实内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性以及总抗氧化能力(T-AOC)均显著升高, 其峰值是对照的1.82~4.56倍, 且在测定时间内始终显著高于对照。同时, BHT处理的果实内丙二醛(MDA)含量变化较小, 最高增幅仅为78.94%, 而接种轮纹病菌的处理, MDA含量急速上升, 最大增幅为316.77%。表明BHT通过持续提高‘富士’果实内防御酶活性和总抗氧化能力, 同时降低膜脂过氧化产物MDA的积累, 从而诱导果实对轮纹病的抗性。

关键词：BHT; 果实轮纹病; 诱导抗性; 防御酶; 丙二醛

收稿：2018-03-01   修定：2018-05-08

资助：山东省重点研发计划(2017CXGC0214)、现代农业产业技术体系建设专项资金(CARS-28)、国家重点研发计划(2016-YFD0201122)、山东省“泰山学者”建设工程专项和青岛农业大学研究生创新立项(QYC201716)。

Control efficiency of BHT against apple fruit ring rot and its influence on the activity of defensive enzymes

YU Chun-Lei, Meng Lu-Lu, LIAN Sen, LI Bao-Hua, LIANG Wen-Xing, WANG Cai-Xia^*

College of Plant Health and Medicine, Key Lab of Integrated Crop Pest Management of Shandong, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, Shangdong 266109, China

Corresponding author: WANG Cai-Xia; E-mail: cxwang@qau.edu.cn

Abstract:

In the present study, apple fruit (Malus domestica) ‘Fuji’ was used to investigate the control efficiency and control mechanism of 2,6-di-tert-butyl-4-methylphenol (BHT) against apple ring rot caused by Botryosphaeria dothidea with the wound inoculation. The results showed that when treated with 0.1 mmol·L^-1 BHT for 48 to 72 h, followed by B. dothidea inoculation, the control efficiency was the best and up to 74%. However, BHT at that concentration had no significant effect on conidial germination and mycelial growth of B. dothidea. The activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) as well as the total antioxidant capacity (T-AOC) were significantly enhanced in apple fruits treated with BHT alone or in combination with B. dothidea, which were all significantly higher than the control in the determination time. The peaks of defense enzyme activity and T-AOC in fruit treated with BHT were 1.82 to 4.56 times higher than those of controls. In addition, malondiadehyde (MDA) content increased less in fruit treated with BHT and the maximum increase rate was only 78.94%, while, MDA content increased sharply and the strongest increase rate was up to 316.77% in fruit inoculated with B. dothidea. The results suggested that BHT could induce apple fruit resistance against B. dothidea infection via increasing the activities of defensive enzymes and T-AOC and reducing the accumulation of MDA.

Key words: BHT; Botryosphaeria dothidea; induced resistance; defensive enzymes; malondiadehyde